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Sequence Characterization and Spatiotemporal Expression Patterns of PbS 26 - RNase Gene in Chinese White Pear (Pyrus bretschneideri)
Joint Authors
Zhang, Lin
Jia, Baoguang
Zou, Feng
Tan, Xiaofeng
Liu, Min
Song, Zhibo
Zeng, Yanling
Jiang, Nan
Yuan, Deyi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Many flowering plants exhibit an important intraspecific reproductive barrier phenomenon, that is, self-incompatibility (SI), in which S-RNase genes play a significant role.
To clarify the specific function of S-RNase genes in Chinese pears, the full length cDNA of PbS 26 - RNase was isolated by rapid amplification of cDNA ends (RACE) technology from Chinese white pear (Pyrus bretschneideri) cultivar “Hongpisu.” The cDNA sequence for PbS 26 - RNase was deposited in GenBank under accession number EU081888.
At the amino acid level, the PbS 26 - RNase displayed the highest similarity (96.9%) with PcSa-RNase of P.
communis, and only seven amino acid differences were present in the two S-RNases.
Phylogenetic analysis of rosaceous S-RNases indicated that the PbS 26 - RNase clustered with maloideous S-RNases, forming a subfamily-specific not a species-specific group.
The PbS 26 - RNase gene was specifically expressed in the style but not other tissues/organs.
The expression level of the PbS 26 - RNase gene rapidly increased at bell balloon stage (BBS), and then it dropped after pollination.
However, the abundance of the PbS 26 - RNase gene transcript in the style was greater after cross-pollination than after self-pollination.
In addition, a method for rapidly detecting the PbS 26 - RNase gene was developed via allele-specific primers design.
The present study could provide a scientific basis for fully clarifying the mechanism of pear SI at the molecular level.
American Psychological Association (APA)
Zhang, Lin& Jia, Baoguang& Zou, Feng& Tan, Xiaofeng& Liu, Min& Song, Zhibo…[et al.]. 2014. Sequence Characterization and Spatiotemporal Expression Patterns of PbS 26 - RNase Gene in Chinese White Pear (Pyrus bretschneideri). The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1048474
Modern Language Association (MLA)
Zhang, Lin…[et al.]. Sequence Characterization and Spatiotemporal Expression Patterns of PbS 26 - RNase Gene in Chinese White Pear (Pyrus bretschneideri). The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1048474
American Medical Association (AMA)
Zhang, Lin& Jia, Baoguang& Zou, Feng& Tan, Xiaofeng& Liu, Min& Song, Zhibo…[et al.]. Sequence Characterization and Spatiotemporal Expression Patterns of PbS 26 - RNase Gene in Chinese White Pear (Pyrus bretschneideri). The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1048474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048474