![](/images/graphics-bg.png)
Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1
Joint Authors
Shuib, Shuwahida
Wan Nawi, Wan Nazatul Naziah
Taha, Ekhlass M.
Omar, Othman
Abdul Kader, Abdul Jalil
Kalil, Mohd Sahaid
Abdul Hamid, Aidil
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Strategic feeding of ammonium and metal ions (Mg2+, Mn2+, Fe3+, Cu2+, Ca2+, Co2+, and Zn2+) for enhanced GLA-rich lipid accumulation in C.
bainieri 2A1 was established.
When cultivated in nitrogen-limited medium, the fungus produced up to 30% lipid (g/g biomass) with 12.9% (g/g lipid) GLA.
However, the accumulation of lipid stopped at 48 hours of cultivation although glucose was abundant.
This event occurred in parallel to the diminishing activity of malic enzyme (ME), fatty acid synthase (FAS), and ATP citrate lyase (ACL) as well as the depletion of metal ions in the medium.
Reinstatement of the enzymes activities was achieved by feeding of ammonium tartrate, but no increment in the lipid content was observed.
However, increment in lipid content from 32% to 50% (g/g biomass) with 13.2% GLA was achieved when simultaneous feeding of ammonium, glucose, and metal ions was carried out.
This showed that the cessation of lipid accumulation was caused by diminishing activities of the enzymes as well as depletion of the metal ions in the medium.
Therefore, strategic feeding of ammonium and metal ions successfully reinstated enzymes activities and enhanced GLA-rich lipid accumulation in C.
bainieri 2A1.
American Psychological Association (APA)
Shuib, Shuwahida& Wan Nawi, Wan Nazatul Naziah& Taha, Ekhlass M.& Omar, Othman& Abdul Kader, Abdul Jalil& Kalil, Mohd Sahaid…[et al.]. 2014. Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1048589
Modern Language Association (MLA)
Shuib, Shuwahida…[et al.]. Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1048589
American Medical Association (AMA)
Shuib, Shuwahida& Wan Nawi, Wan Nazatul Naziah& Taha, Ekhlass M.& Omar, Othman& Abdul Kader, Abdul Jalil& Kalil, Mohd Sahaid…[et al.]. Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1048589
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048589