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Production, Characterization of Tannase from Penicillium montanense URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (Vitis vinifera L.)
Joint Authors
Lima, Juliana Silva de
Cruz, Roberta
Fonseca, Julyanna Cordoville
Medeiros, Erika Valente de
Maciel, Marília de Holanda Cavalcanti
Moreira, Keila Aparecida
Motta, Cristina Maria de Souza
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Tannase is an enzyme that hydrolyzes esters and lateral bonds of tannins, such as tannic acid, releasing glucose and gallic acid and stands out in the clarification of wines and juices.
Fungi of the genera Aspergillus and Penicillium are excellent producers of this enzyme.
The search for fungi that produce high levels of tannase as well as new substrates for the enzyme production by the SSF is required.
The objectives of this study were to evaluate the production of tannase by Aspergillus and Penicillium species through SSF using leaves and agroindustrial waste barbados cherry and mangaba fruit as substrate, select the best producer, optimize production, characterize the crude enzyme extract, and apply it the clarification of grape juice.
Selecting the best producer was performed by planning Placket-Burman and RSM.
P.
montanense showed highest activity with 41.64 U/mL after 72 h of fermentation residue using barbados cherry, with 3.5% tannic acid and 70% moisture.
The enzyme showed the highest activity at pH 9.0 and 50°C.
The tannase of P.
montanense was stable over a wide pH range and temperature and, when applied to grape juice, showed higher efficiency by reducing 46% of the tannin content after incubation 120 m.
American Psychological Association (APA)
Lima, Juliana Silva de& Cruz, Roberta& Fonseca, Julyanna Cordoville& Medeiros, Erika Valente de& Maciel, Marília de Holanda Cavalcanti& Moreira, Keila Aparecida…[et al.]. 2014. Production, Characterization of Tannase from Penicillium montanense URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (Vitis vinifera L.). The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1048627
Modern Language Association (MLA)
Lima, Juliana Silva de…[et al.]. Production, Characterization of Tannase from Penicillium montanense URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (Vitis vinifera L.). The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1048627
American Medical Association (AMA)
Lima, Juliana Silva de& Cruz, Roberta& Fonseca, Julyanna Cordoville& Medeiros, Erika Valente de& Maciel, Marília de Holanda Cavalcanti& Moreira, Keila Aparecida…[et al.]. Production, Characterization of Tannase from Penicillium montanense URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (Vitis vinifera L.). The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1048627
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048627