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Biobleaching of Industrial Important Dyes with Peroxidase Partially Purified from Garlic
Joint Authors
Eze, Sabinus O. O.
Osuji, Akudo Chigozirim
Osayi, Emmanuel Emeka
Chilaka, Ferdinand Chiemeka
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
An acidic peroxidase was extracted from garlic (Allium sativum) and was partially purified threefold by ammonium sulphate precipitation, dialysis, and gel filtration chromatography using sephadex G-200.
The specific activity of the enzyme increased from 4.89 U/mg after ammonium sulphate precipitation to 25.26 U/mg after gel filtration chromatography.
The optimum temperature and pH of the enzyme were 50°C and 5.0, respectively.
The Km and V max for H2O2 and o-dianisidine were 0.026 mM and 0.8 U/min, and 25 mM and 0.75 U/min, respectively.
Peroxidase from garlic was effective in decolourizing Vat Yellow 2, Vat Orange 11, and Vat Black 27 better than Vat Green 9 dye.
For all the parameters monitored, the decolourization was more effective at a pH range, temperature, H2O2 concentration, and enzyme concentration of 4.5–5.0, 50°C, 0.6 mM, and 0.20 U/mL, respectively.
The observed properties of the enzyme together with its low cost of extraction (from local sources) show the potential of this enzyme for practical application in industrial wastewater treatment especially with hydrogen peroxide.
These Vat dyes also exhibited potentials of acting as peroxidase inhibitors at alkaline pH range.
American Psychological Association (APA)
Osuji, Akudo Chigozirim& Eze, Sabinus O. O.& Osayi, Emmanuel Emeka& Chilaka, Ferdinand Chiemeka. 2014. Biobleaching of Industrial Important Dyes with Peroxidase Partially Purified from Garlic. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1048633
Modern Language Association (MLA)
Osuji, Akudo Chigozirim…[et al.]. Biobleaching of Industrial Important Dyes with Peroxidase Partially Purified from Garlic. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1048633
American Medical Association (AMA)
Osuji, Akudo Chigozirim& Eze, Sabinus O. O.& Osayi, Emmanuel Emeka& Chilaka, Ferdinand Chiemeka. Biobleaching of Industrial Important Dyes with Peroxidase Partially Purified from Garlic. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1048633
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048633