Characterisation of Drosophila UbxCPTI000601 and hthCPTI000378 Protein Trap Lines
Joint Authors
Choo, Siew Woh
Beh, Ching Yew
Russell, Steven
White, Robert
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-15
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
In Drosophila, protein trap strategies provide powerful approaches for the generation of tagged proteins expressed under endogenous control.
Here, we describe expression and functional analysis to evaluate new Ubx and hth protein trap lines generated by the Cambridge Protein Trap project.
Both protein traps exhibit spatial and temporal expression patterns consistent with the reported endogenous pattern in the embryo.
In imaginal discs, Ubx-YFP is expressed throughout the haltere and 3rd leg imaginal discs, while Hth-YFP is expressed in the proximal regions of haltere and wing discs but not in the pouch region.
The UbxCPTI000601 line is semilethal as a homozygote.
No T3/A1 to T2 transformations were observed in the embryonic cuticle or the developing midgut.
The homozygous survivors, however, exhibit a weak haltere phenotype with a few wing-like marginal bristles on the haltere capitellum.
Although hthCPTI000378 is completely lethal as a homozygote, the hthCPTI000378/hthC1 genotype is viable.
Using a hth deletion (Df(3R)BSC479) we show that hthCPTI000378/Df(3R)BSC479 adults are phenotypically normal.
No transformations were observed in hthCPTI000378, hthCPTI000378/hthC1, or hthCPTI000378/Df(3R)BSC479 embryonic cuticles.
We have successfully characterised the Ubx-YFP and Hth-YFP protein trap lines demonstrating that the tagged proteins show appropriate expression patterns and produce at least partially functional proteins.
American Psychological Association (APA)
Choo, Siew Woh& Beh, Ching Yew& Russell, Steven& White, Robert. 2014. Characterisation of Drosophila UbxCPTI000601 and hthCPTI000378 Protein Trap Lines. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1048667
Modern Language Association (MLA)
Choo, Siew Woh…[et al.]. Characterisation of Drosophila UbxCPTI000601 and hthCPTI000378 Protein Trap Lines. The Scientific World Journal No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1048667
American Medical Association (AMA)
Choo, Siew Woh& Beh, Ching Yew& Russell, Steven& White, Robert. Characterisation of Drosophila UbxCPTI000601 and hthCPTI000378 Protein Trap Lines. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1048667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048667