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RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter
Joint Authors
Moreno-Maldonado, Rodolfo
Murillas, Rodolfo
Navarro, Manuel
Page, Angustias
Suarez-Cabrera, Cristian
Alameda, Josefa P.
Bravo, Ana
Casanova, M. Llanos
Ramirez, Angel
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Inhibition of gene expression through siRNAs is a tool increasingly used for the study of gene function in model systems, including transgenic mice.
To achieve perdurable effects, the stable expression of siRNAs by an integrated transgenic construct is necessary.
For transgenic siRNA expression, promoters transcribed by either RNApol II or III (such as U6 or H1 promoters) can be used.
Relatively large amounts of small RNAs synthesis are achieved when using RNApol III promoters, which can be advantageous in knockdown experiments.
To study the feasibility of H1 promoter-driven RNAi-expressing constructs for protein knockdown in transgenic mice, we chose IKK1 as the target gene.
Our results indicate that constructs containing the H1 promoter are sensitive to the presence of prokaryotic sequences and to transgene position effects, similar to RNApol II promoters-driven constructs.
We observed variable expression levels of transgenic siRNA among different tissues and animals and a reduction of up to 80% in IKK1 expression.
Furthermore, IKK1 knockdown led to hair follicle alterations.
In summary, we show that constructs directed by the H1 promoter can be used for knockdown of genes of interest in different organs and for the generation of animal models complementary to knockout and overexpression models.
American Psychological Association (APA)
Moreno-Maldonado, Rodolfo& Murillas, Rodolfo& Navarro, Manuel& Page, Angustias& Suarez-Cabrera, Cristian& Alameda, Josefa P.…[et al.]. 2014. RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1048677
Modern Language Association (MLA)
Moreno-Maldonado, Rodolfo…[et al.]. RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1048677
American Medical Association (AMA)
Moreno-Maldonado, Rodolfo& Murillas, Rodolfo& Navarro, Manuel& Page, Angustias& Suarez-Cabrera, Cristian& Alameda, Josefa P.…[et al.]. RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1048677
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048677