In Vitro Conservation of Sweet Potato Genotypes
Joint Authors
Arrigoni-Blank, Maria de Fátima
Tavares, Fernanda Ferreira
Blank, Arie Fitzgerald
Santos, Maria Clézia dos
Menezes, Thays Saynara Alves
Santana, Aléa Dayane Dantas de
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The aim of this study was to develop a protocol for the in vitro conservation of sweet potato genotypes using the slow growth technique.
The first experiment was conducted in a 4×5×2 factorial scheme, testing four genotypes (IPB-007, IPB-052, IPB-072, and IPB-137), five concentrations of abscisic acid (ABA) (0.0, 1.0, 2.0, 4.0, and 8.0 mg·L−1), and two temperatures (18 and 25°C).
The second experiment was conducted in a 4×3×3 factorial scheme at 18°C, testing four genotypes (IPB-007, IPB-052, IPB-072, and IPB-137), three variations of MS salts (50, 75, and 100%), and three concentrations of sucrose (10, 20, and 30 g·L−1).
Every three months, we evaluated the survival (%), shoot height, and shoot viability.
In vitro conservation of the sweet potato genotypes IPB-052 and IPB-007 was obtained over three and six months, respectively, using MS medium plus 2.0 mg·L−1 of ABA at either 18 or 25°C.
Genotypes IPB-072 and IPB-137 can be kept for three and six months, respectively, in MS medium without ABA at 18°C.
It is possible to store IPB-052 and IPB-072 for six months and IPB-007 and IPB-137 for nine months using 30 g·L−1 of sucrose and 50% MS salts.
American Psychological Association (APA)
Arrigoni-Blank, Maria de Fátima& Tavares, Fernanda Ferreira& Blank, Arie Fitzgerald& Santos, Maria Clézia dos& Menezes, Thays Saynara Alves& Santana, Aléa Dayane Dantas de. 2014. In Vitro Conservation of Sweet Potato Genotypes. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1048735
Modern Language Association (MLA)
Arrigoni-Blank, Maria de Fátima…[et al.]. In Vitro Conservation of Sweet Potato Genotypes. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1048735
American Medical Association (AMA)
Arrigoni-Blank, Maria de Fátima& Tavares, Fernanda Ferreira& Blank, Arie Fitzgerald& Santos, Maria Clézia dos& Menezes, Thays Saynara Alves& Santana, Aléa Dayane Dantas de. In Vitro Conservation of Sweet Potato Genotypes. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1048735
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048735