Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences
Joint Authors
Park, Seong Hwan
Ko, Kwang Soo
Jo, Tae-Ho
Hwang, Juck-Joon
Kim, Yu-Hoon
Shin, Sang Eon
Ham, Chan Seon
Kim, Seong Yoon
Son, Gi Hoon
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Identification of insect species is an important task in forensic entomology.
For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized.
We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea.
After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed.
Obtained sequences were analyzed for a phylogenetic tree and a distance matrix.
Our data showed very low intraspecific sequence distances and species-level monophylies.
However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation.
To the best of our knowledge, this study is the first report of COI nucleotide sequences from Hydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, and Phaonia aureola.
American Psychological Association (APA)
Kim, Yu-Hoon& Shin, Sang Eon& Ham, Chan Seon& Kim, Seong Yoon& Ko, Kwang Soo& Jo, Tae-Ho…[et al.]. 2014. Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049024
Modern Language Association (MLA)
Kim, Yu-Hoon…[et al.]. Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1049024
American Medical Association (AMA)
Kim, Yu-Hoon& Shin, Sang Eon& Ham, Chan Seon& Kim, Seong Yoon& Ko, Kwang Soo& Jo, Tae-Ho…[et al.]. Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049024