Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology
Joint Authors
Vijayaraghavan, Ponnuswamy
Prakash Vincent, Samuel Gnana
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Production of fibrinolytic enzyme by a newly isolated Paenibacillus sp.
IND8 was optimized using wheat bran in solid state fermentation.
A 25 full factorial design (first-order model) was applied to elucidate the key factors as moisture, pH, sucrose, yeast extract, and sodium dihydrogen phosphate.
Statistical analysis of the results has shown that moisture, sucrose, and sodium dihydrogen phosphate have the most significant effects on fibrinolytic enzymes production ( P < 0.05 ).
Central composite design (CCD) was used to determine the optimal concentrations of these three components and the experimental results were fitted with a second-order polynomial model at 95% level ( P < 0.05 ).
Overall, 4.5-fold increase in fibrinolytic enzyme production was achieved in the optimized medium as compared with the unoptimized medium.
American Psychological Association (APA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. 2014. Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049035
Modern Language Association (MLA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1049035
American Medical Association (AMA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049035