Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.)
Joint Authors
Mahmood Ibrahim, Ahmed
Binti Kayat, Fatimah
Ermiena Surya Mat Hussin, Zeti
Susanto, Dwi
Ariffulah, Mohammed
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-13
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber.
Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding.
The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material.
In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied.
Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus.
The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation.
At that stage, the microspores were at the uninucleate stage which was suitable for culture.
The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.
American Psychological Association (APA)
Mahmood Ibrahim, Ahmed& Binti Kayat, Fatimah& Ermiena Surya Mat Hussin, Zeti& Susanto, Dwi& Ariffulah, Mohammed. 2014. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.). The Scientific World Journal،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1049069
Modern Language Association (MLA)
Mahmood Ibrahim, Ahmed…[et al.]. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.). The Scientific World Journal No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1049069
American Medical Association (AMA)
Mahmood Ibrahim, Ahmed& Binti Kayat, Fatimah& Ermiena Surya Mat Hussin, Zeti& Susanto, Dwi& Ariffulah, Mohammed. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.). The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1049069
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049069