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Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects
Joint Authors
Sumathi, C.
MohanaPriya, D.
Swarnalatha, S.
Dinesh, M. G.
Sekaran, Ganesan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Aim.
The focal theme of present investigation includes isolation of prodigiosin producing fish gut bacteria, enhancing its production using tannery solid waste fleshing, and evaluation of its pharmacological effect.
Methods.
Optimization of fermentation conditions to yield maximum prodigiosin, and instrumental analysis using FTIR, NMR, ESI-MS, TGA, and DSC.
Results.
The optimum conditions required for the maximum prodigiosin concentration were achieved at time 30 h, temperature 30°C, pH 8, and 3% substrate concentration.
The secondary metabolite was analyzed using ESI-MS, FTIR, and NMR.
Therapeutic efficacy was assessed by in vitro anticancer studies.
Among the pathogenic bacteria Pseudomonas aeruginosa was most susceptible at the lowest concentration followed by Salmonella typhi.
IC50 concentration was cell line specific (HeLa cells: 4.3 µM, HEp2: 5.2 µM, and KB cells: 4.8 µM) and remains nontoxic up to the concentration of 25 µM on normal Vero cells suggesting that cancerous cells are more susceptible to the prodigiosin at lower concentration.
Conclusion.
Maximum prodigiosin production was obtained with tannery fleshing.
The potency of the fish gut bacterial secondary metabolite prodigiosin as a therapeutic agent was confirmed through in vitro antimicrobial and anticancer studies.
American Psychological Association (APA)
Sumathi, C.& MohanaPriya, D.& Swarnalatha, S.& Dinesh, M. G.& Sekaran, Ganesan. 2014. Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049098
Modern Language Association (MLA)
Sumathi, C.…[et al.]. Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1049098
American Medical Association (AMA)
Sumathi, C.& MohanaPriya, D.& Swarnalatha, S.& Dinesh, M. G.& Sekaran, Ganesan. Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049098