Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations
Joint Authors
Zhou, Dong-Hui
Xu, Ying
Zhang, Nian-Zhang
Huang, Si-Yang
Wang, Rui-Ai
Zhu, Xing-Quan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-10
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Genetic diversity of T.
gondii is a concern of many studies, due to the biological and epidemiological diversity of this parasite.
The present study examined sequence variation in rhoptry protein 17 (ROP17) gene among T.
gondii isolates from different hosts and geographical regions.
The rop17 gene was amplified and sequenced from 10 T.
gondii strains, and phylogenetic relationship among these T.
gondii strains was reconstructed using maximum parsimony (MP), neighbor-joining (NJ), and maximum likelihood (ML) analyses.
The partial rop17 gene sequences were 1375 bp in length and A+T contents varied from 49.45% to 50.11% among all examined T.
gondii strains.
Sequence analysis identified 33 variable nucleotide positions (2.1%), 16 of which were identified as transitions.
Phylogeny reconstruction based on rop17 gene data revealed two major clusters which could readily distinguish Type I and Type II strains.
Analyses of sequence variations in nucleotides and amino acids among these strains revealed high ratio of nonsynonymous to synonymous polymorphisms (>1), indicating that rop17 shows signs of positive selection.
This study demonstrated the existence of slightly high sequence variability in the rop17 gene sequences among T.
gondii strains from different hosts and geographical regions, suggesting that rop17 gene may represent a new genetic marker for population genetic studies of T.
gondii isolates.
American Psychological Association (APA)
Zhang, Nian-Zhang& Xu, Ying& Huang, Si-Yang& Zhou, Dong-Hui& Wang, Rui-Ai& Zhu, Xing-Quan. 2014. Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-1049285
Modern Language Association (MLA)
Zhang, Nian-Zhang…[et al.]. Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations. The Scientific World Journal No. 2014 (2014), pp.1-4.
https://search.emarefa.net/detail/BIM-1049285
American Medical Association (AMA)
Zhang, Nian-Zhang& Xu, Ying& Huang, Si-Yang& Zhou, Dong-Hui& Wang, Rui-Ai& Zhu, Xing-Quan. Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-1049285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049285