Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane
Joint Authors
Kahl, Günter
Ferreira Neto, José Ribamar Costa
Nardi, Claudia Huerta de
Benko-Iseppon, Ana Maria
Silva, Roberta Lane de Oliveira
Kido, Éderson Akio
Chabregas, Sabrina Moutinho
da Silva, Manassés Daniel
Burnquist, William Lee
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
One of the most challenging aspects of RT-qPCR data analysis is the identification of reliable reference genes.
Ideally, they should be neither induced nor repressed under different experimental conditions.
To date, few reference genes have been adequately studied for sugarcane (Saccharum spp.) using statistical approaches.
In this work, six candidate genes (αTUB, GAPDH, H1, SAMDC, UBQ, and 25S rRNA) were tested for gene expression normalization of sugarcane root tissues from drought-tolerant and -sensitive accessions after continuous dehydration (24 h).
By undergoing different approaches (GeNorm, NormFinder, and BestKeeper), it was shown that most of them could be used in combinations for normalization purposes, with the exception of SAMDC.
Nevertheless three of them (H1, αTUB, and GAPDH) were considered the most reliable reference genes.
Their suitability as reference genes validated the expression profiles of two targets (AS and PFPα1), related to SuperSAGE unitags, in agreement with results revealed by previous in silico analysis.
The other two sugarcane unitags (ACC oxidase and PIP1-1), after salt stress (100 mM NaCl), presented their expressions validated in the same way.
In conclusion, these reference genes will be useful for dissecting gene expression in sugarcane roots under abiotic stress, especially in transcriptomic studies using SuperSAGE or RNAseq approaches.
American Psychological Association (APA)
Silva, Roberta Lane de Oliveira& da Silva, Manassés Daniel& Ferreira Neto, José Ribamar Costa& Nardi, Claudia Huerta de& Chabregas, Sabrina Moutinho& Burnquist, William Lee…[et al.]. 2014. Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1049312
Modern Language Association (MLA)
Silva, Roberta Lane de Oliveira…[et al.]. Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane. The Scientific World Journal No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1049312
American Medical Association (AMA)
Silva, Roberta Lane de Oliveira& da Silva, Manassés Daniel& Ferreira Neto, José Ribamar Costa& Nardi, Claudia Huerta de& Chabregas, Sabrina Moutinho& Burnquist, William Lee…[et al.]. Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1049312
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049312