Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes
Joint Authors
Liao, Wei-Ting
Huang, Bin
Chiang, Kuo-Hao
Yu, Hsin-Su
Chen, Ying-Lun
You, Huey-Ling
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Arsenic is a class I human carcinogen (such as inducing skin cancer) by its prominent chemical interaction with protein thio (-SH) group.
Therefore, arsenic may compromise protein S-nitrosylation by competing the -SH binding activity.
In the present study, we aimed to understand the influence of arsenic on protein S-nitrosylation and the following proteomic changes.
By using primary human skin keratinocyte, we found that arsenic treatment decreased the level of protein S-nitrosylation.
This was coincident to the decent expressions of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS).
By using LC-MS/MS, around twenty S-nitrosoproteins were detected in the biotin-switched eluent.
With the interest that arsenic not only regulates posttranslational S-nitrosylation but also separately affects protein’s translation expression, we performed two-dimensional gel electrophoresis and found that 8 proteins were significantly decreased during arsenic treatment.
Whether these decreased proteins are the consequence of protein S-nitrosylation will be further investigated.
Taken together, these results provide a finding that arsenic can deplete the binding activity of NO and therefore reduce protein S-nitrosylation.
American Psychological Association (APA)
Huang, Bin& Chiang, Kuo-Hao& Yu, Hsin-Su& Chen, Ying-Lun& You, Huey-Ling& Liao, Wei-Ting. 2014. Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1049324
Modern Language Association (MLA)
Huang, Bin…[et al.]. Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1049324
American Medical Association (AMA)
Huang, Bin& Chiang, Kuo-Hao& Yu, Hsin-Su& Chen, Ying-Lun& You, Huey-Ling& Liao, Wei-Ting. Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1049324
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049324