Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
Joint Authors
Gojkovic, Živan
Vílchez, Carlos
Torronteras, Rafael
Vigara, Javier
Gómez-Jacinto, Veronica
Janzer, Nora
Gómez-Ariza, José-Luis
Márová, Ivana
Garbayo, Ines
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures.
Culture exposed to sublethal Se concentrations of 40 mg·L−1 (212 μM) decreased growth rates for about 25% compared to control.
A selenate EC50 value of 45 mg·L−1 (238.2 μM) was determined.
Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half.
Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity.
Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA.
Results revealed that the microalga was able to accumulate up to 140 mg·kg−1 of SeMet in 120 h of cultivation.
This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.
American Psychological Association (APA)
Gojkovic, Živan& Vílchez, Carlos& Torronteras, Rafael& Vigara, Javier& Gómez-Jacinto, Veronica& Janzer, Nora…[et al.]. 2014. Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1049470
Modern Language Association (MLA)
Gojkovic, Živan…[et al.]. Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture. The Scientific World Journal No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1049470
American Medical Association (AMA)
Gojkovic, Živan& Vílchez, Carlos& Torronteras, Rafael& Vigara, Javier& Gómez-Jacinto, Veronica& Janzer, Nora…[et al.]. Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1049470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049470