![](/images/graphics-bg.png)
The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis
Joint Authors
Taha, Rosna Mat
Farzinebrahimi, Reza
Rashid, Kamaludin
Syafawati Yaacob, Jamilah
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1 with 0.5 increment).
After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L−1) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L−1) respectively were used.
Leaf segments cultured on WPM media added with Kn (1 mg L−1) and TDZ (2 mg L−1) yielded the least amount of callus.
It was found that WPM media added with IAA (4.5–5.0 mg L−1) were optimum for root induction from G.
jasminoides plantlets.
Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect against E.
coli, P.
aeruginosa, S.
aureus, and B.
cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity against E.
coli and B.
cereus.
The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts.
American Psychological Association (APA)
Farzinebrahimi, Reza& Taha, Rosna Mat& Rashid, Kamaludin& Syafawati Yaacob, Jamilah. 2014. The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1049502
Modern Language Association (MLA)
Farzinebrahimi, Reza…[et al.]. The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1049502
American Medical Association (AMA)
Farzinebrahimi, Reza& Taha, Rosna Mat& Rashid, Kamaludin& Syafawati Yaacob, Jamilah. The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1049502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049502