Response Surface Methodology: Optimisation of Antifungal Bioemulsifier from Novel Bacillus thuringiensis
Joint Authors
Ponnusami, V.
Rajendran, Deepak
Ramakrishnan, Jayapradha
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310.
To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production.
The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification.
A quadratic equation was employed to express the response variable in terms of the independent variables.
Statistical tools like student’s t -test, F -test, and ANOVA were employed to identify the important factors and to test the adequacy of the model.
Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.
American Psychological Association (APA)
Rajendran, Deepak& Ponnusami, V.& Ramakrishnan, Jayapradha. 2014. Response Surface Methodology: Optimisation of Antifungal Bioemulsifier from Novel Bacillus thuringiensis. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049580
Modern Language Association (MLA)
Rajendran, Deepak…[et al.]. Response Surface Methodology: Optimisation of Antifungal Bioemulsifier from Novel Bacillus thuringiensis. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1049580
American Medical Association (AMA)
Rajendran, Deepak& Ponnusami, V.& Ramakrishnan, Jayapradha. Response Surface Methodology: Optimisation of Antifungal Bioemulsifier from Novel Bacillus thuringiensis. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049580