Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila
Joint Authors
Rathore, Jitendra Singh
Gautam, Lalit Kumar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Bacterial toxin-antitoxin (TA) complexes induce programmed cell death and also function to relieve cell from stress by various response mechanisms.
Escherichia coli RelB-RelE TA complex consists of a RelE toxin functionally counteracted by RelB antitoxin.
In the present study, a novel homolog of RelE toxin designated as Xn-relE toxin from Xenorhabdus nematophila possessing its own antitoxin designated as Xn-relEAT has been identified.
Expression and purification of recombinant proteins under native conditions with GST and Ni-NTA chromatography prove the existence of novel TA module.
The expression of recombinant Xn-relE under tightly regulated ara promoter in E.
coli Top 10 cells confirms its toxic nature in endogenous toxicity assay.
The neutralization activity in endogenous toxicity assay by Xn-relEAT antitoxin confirms its antidote nature when studying the whole TA operon under ara regulated promoter.
This study promotes newly discovered TA module to be regarded as important as other proteins of type II toxin-antitoxin system.
American Psychological Association (APA)
Rathore, Jitendra Singh& Gautam, Lalit Kumar. 2014. Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1049597
Modern Language Association (MLA)
Rathore, Jitendra Singh& Gautam, Lalit Kumar. Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1049597
American Medical Association (AMA)
Rathore, Jitendra Singh& Gautam, Lalit Kumar. Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1049597
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049597