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Synergistic Antitumor Effect of Doxorubicin and Tacrolimus (FK506) on Hepatocellular Carcinoma Cell Lines
Joint Authors
Capone, Francesca
Guerriero, Eliana
Sorice, Angela
Colonna, Giovanni
Storti, Gabriella
Pagliuca, Jessica
Castello, Giuseppe
Costantini, Susan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Hepatocellular carcinoma is the fifth most common cancer worldwide and shows a complex clinical course, poor response to pharmacological treatment, and a severe prognosis.
Thus, the aim of this study was to investigate whether tacrolimus (FK506) has synergistic antitumor effects with doxorubicin on two human hepatocellular carcinoma cell lines, Huh7 and HepG2.
Cell viability was analyzed by Sulforhodamine B assay and synergic effect was evaluated by the software CalcuSyn.
Cell apoptosis was evaluated using Annexin V and Dead Cell assay.
Apoptosis-related protein PARP-1 cleaved and autophagy-related protein expressions (Beclin-1 and LC3B) were measured by western blotting analysis.
Cytokines concentration in cellular supernatants after treatments was studied by Bio-Plex assay.
Interestingly the formulation with doxorubicin and tacrolimus induced higher cytotoxicity level on tumor cells than single treatment.
Moreover, our results showed that the mechanisms involved were (i) a strong cell apoptosis induction, (ii) contemporaneous decrease of autophagy activation, understood as prosurvival process, and (iii) downregulation of proinflammatory cytokines.
In conclusion, future studies could relate to the doxorubicin/tacrolimus combination effects in mice models bearing HCC in order to see if this formulation could be useful in HCC treatment.
American Psychological Association (APA)
Capone, Francesca& Guerriero, Eliana& Sorice, Angela& Colonna, Giovanni& Storti, Gabriella& Pagliuca, Jessica…[et al.]. 2014. Synergistic Antitumor Effect of Doxorubicin and Tacrolimus (FK506) on Hepatocellular Carcinoma Cell Lines. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049653
Modern Language Association (MLA)
Capone, Francesca…[et al.]. Synergistic Antitumor Effect of Doxorubicin and Tacrolimus (FK506) on Hepatocellular Carcinoma Cell Lines. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1049653
American Medical Association (AMA)
Capone, Francesca& Guerriero, Eliana& Sorice, Angela& Colonna, Giovanni& Storti, Gabriella& Pagliuca, Jessica…[et al.]. Synergistic Antitumor Effect of Doxorubicin and Tacrolimus (FK506) on Hepatocellular Carcinoma Cell Lines. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049653
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049653