Evaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines
Joint Authors
Arora, Shagun
Tandon, Simran
Tandon, Chanderdeep
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The purpose of this current study was to justify the incorporation of complementary and alternate medicine (CAM) in current cancer treatments.
The major drawback of anticancer drugs is their nonselective killing, which ultimately leads to attrition of normal cells.
Keeping this as the foundation of our study, we made an effort to compare the cytotoxicity associated with a known chemotherapeutic drug 5-Fluorouracil (5-FU), with certain CAM therapies previously reported to have anticancer activity.
The parameters chosen for the study were based on antiproliferative and cytotoxic effects on normal, kidney epithelial cells (NRK-52E).
The MTT assay, colony formation assay, DNA fragmentation, and differential staining using AO/EB, following treatment with either 5-FU or CAM therapies, were performed.
The CAM therapies under study were various extracts of wheatgrass, roots of Achyranthes aspera (AA), mushroom extracts (Pleurotus ostreatus, Macrolepiota procera, and Auricularia polytricha), and a homeopathic drug, Ruta graveolens (Ruta).
The results showed that treatment of normal cells with the CAM therapies led to minimum cell damage in comparison to 5-FU.
This evidence-based study will lead to greater acceptance of alternative therapies against cancer.
American Psychological Association (APA)
Arora, Shagun& Tandon, Chanderdeep& Tandon, Simran. 2014. Evaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1049668
Modern Language Association (MLA)
Arora, Shagun…[et al.]. Evaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1049668
American Medical Association (AMA)
Arora, Shagun& Tandon, Chanderdeep& Tandon, Simran. Evaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1049668
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049668