![](/images/graphics-bg.png)
Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2
Joint Authors
Lucena, Eudes Euler de Souza
Guzen, Fausto Pierdoná
Cavalcanti, José Rodolfo Lopes de Paiva
Marinho, Maria Jocileide de Medeiros
Pereira, Wogelsanger Oliveira
Barboza, Carlos Augusto Galvão
Cavalcante, Jeferson Sousa
Nascimento Junior, Expedito S.
Costa, Miriam Stela M. O.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs).
The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential.
This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2).
The growth and morphology were assessed for over 72 hours.
Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200).
Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4.
Cells cultivated only with conditioned medium acquired a glial phenotype.
Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200.
This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.
American Psychological Association (APA)
Lucena, Eudes Euler de Souza& Guzen, Fausto Pierdoná& Cavalcanti, José Rodolfo Lopes de Paiva& Marinho, Maria Jocileide de Medeiros& Pereira, Wogelsanger Oliveira& Barboza, Carlos Augusto Galvão…[et al.]. 2014. Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049682
Modern Language Association (MLA)
Lucena, Eudes Euler de Souza…[et al.]. Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1049682
American Medical Association (AMA)
Lucena, Eudes Euler de Souza& Guzen, Fausto Pierdoná& Cavalcanti, José Rodolfo Lopes de Paiva& Marinho, Maria Jocileide de Medeiros& Pereira, Wogelsanger Oliveira& Barboza, Carlos Augusto Galvão…[et al.]. Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1049682
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049682