![](/images/graphics-bg.png)
NMDA Receptor-Mediated Neuroprotective Effect of the Scutellaria baicalensis Georgi Extract on the Excitotoxic Neuronal Cell Death in Primary Rat Cortical Cell Cultures
Joint Authors
Yang, Jinsong
Wu, Xiaohong
Yu, Haogang
Liao, Xinbiao
Teng, Lisong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The objective of the current research work was to evaluate the neuroprotective effect of the ethanol extract of Scutellaria baicalensis (S.B.) on the excitotoxic neuronal cell death in primary rat cortical cell cultures.
The inhibitory effects of the extract were qualitatively and quantitatively estimated by phase-contrast microscopy and lactate dehydrogenase (LDH) assays.
The extract exhibited a potent and dose-dependent inhibition of the glutamate-induced excitotoxicity in the culture media.
Further, using radioligand binding assays, it was observed that the inhibitory effect of the extract was more potent and selective for the N-methyl-D-aspartate (NMDA) receptor-mediated toxicity.
The S.B.
ethanol extract competed with [3H] MDL 105,519 for the specific binding to the NMDA receptor glycine site with 50% inhibition occurring at 35.1 μg/mL.
Further, NMDA receptor inactivation by the S.B.
ethanol extract was concluded from the decreasing binding capability of [3H]MK-801 in the presence of the extract.
Thus, S.B.
extract exhibited neuroprotection against excitotoxic cell death, and this neuroprotection was mediated through the inhibition of NMDA receptor function by interacting with the glycine binding site of the NMDA receptor.
Phytochemical analysis of the bioactive extract revealed the presence of six phytochemical constituents including baicalein, baicalin, wogonin, wogonoside, scutellarin, and Oroxylin A.
American Psychological Association (APA)
Yang, Jinsong& Wu, Xiaohong& Yu, Haogang& Liao, Xinbiao& Teng, Lisong. 2014. NMDA Receptor-Mediated Neuroprotective Effect of the Scutellaria baicalensis Georgi Extract on the Excitotoxic Neuronal Cell Death in Primary Rat Cortical Cell Cultures. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049695
Modern Language Association (MLA)
Yang, Jinsong…[et al.]. NMDA Receptor-Mediated Neuroprotective Effect of the Scutellaria baicalensis Georgi Extract on the Excitotoxic Neuronal Cell Death in Primary Rat Cortical Cell Cultures. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1049695
American Medical Association (AMA)
Yang, Jinsong& Wu, Xiaohong& Yu, Haogang& Liao, Xinbiao& Teng, Lisong. NMDA Receptor-Mediated Neuroprotective Effect of the Scutellaria baicalensis Georgi Extract on the Excitotoxic Neuronal Cell Death in Primary Rat Cortical Cell Cultures. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049695