Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development
Joint Authors
Nieuwesteeg, M. A.
Willson, J. A.
Cepeda, M.
Fox, M. A.
Damjanovski, S.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Extracellular matrix (ECM) remodeling is essential for facilitating developmental processes.
ECM remodeling, accomplished by matrix metalloproteinases (MMPs), is regulated by endogenous tissue inhibitors of metalloproteinases (TIMPs).
While the TIMP N-terminal domain is involved in inhibition of MMP activity, the C-terminal domain exhibits cell-signaling activity, which is TIMP and cell type dependent.
We have previously examined the distinct roles of the Xenopus laevis TIMP-2 and -3 C-terminal domains during development and here examined the unique roles of TIMP-1 N- and C-terminal domains in early X.
laevis embryos.
mRNA microinjection was used to overexpress full-length TIMP-1 or its individual N- or C-terminal domains in embryos.
Full-length and C-terminal TIMP-1 resulted in increased lethality compared to N-terminal TIMP-1.
Overexpression of C-terminal TIMP-1 resulted in significant decreases in mRNA levels of proteolytic genes including TIMP-2, RECK, MMP-2, and MMP-9, corresponding to decreases in MMP-2 and -9 protein levels, as well as decreased MMP-2 and MMP-9 activities.
These trends were not observed with the N-terminus.
Our research suggests that the individual domains of TIMP-1 are capable of playing distinct roles in regulating the ECM proteolytic network during development and that the unique functions of these domains are moderated in the endogenous full-length TIMP-1 molecule.
American Psychological Association (APA)
Nieuwesteeg, M. A.& Willson, J. A.& Cepeda, M.& Fox, M. A.& Damjanovski, S.. 2014. Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1049725
Modern Language Association (MLA)
Nieuwesteeg, M. A.…[et al.]. Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development. The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1049725
American Medical Association (AMA)
Nieuwesteeg, M. A.& Willson, J. A.& Cepeda, M.& Fox, M. A.& Damjanovski, S.. Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1049725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049725