Inhibitory Effect of Cinobufagin on L-Type Ca2+ Currents, Contractility, and Ca2+ Homeostasis of Isolated Adult Rat Ventricular Myocytes
Joint Authors
Li, Pinya
Song, Qiongtao
Liu, Tao
Wu, Zhonglin
Chu, Xi
Zhang, Xuan
Zhang, Ying
Gao, Yonggang
Zhang, Jianping
Chu, Li
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Cinobufagin (CBG), a major bioactive ingredient of the bufanolide steroid compounds of Chan Su, has been widely used to treat coronary heart disease.
At present, the effect of CBG on the L-type Ca2+ current (ICa-L) of ventricular myocytes remains undefined.
The aim of the present study was to characterize the effect of CBG on intracellular Ca2+ ([Ca2+]i) handling and cell contractility in rat ventricular myocytes.
CBG was investigated by determining its influence on ICa-L, Ca2+ transient, and contractility in rat ventricular myocytes using the whole-cell patch-clamp technique and video-based edge-detection and dual-excitation fluorescence photomultiplier systems.
The dose of CBG (10−8 M) decreased the maximal inhibition of CBG by 47.93%.
CBG reduced ICa-L in a concentration-dependent manner with an IC50 of 4 × 10−10 M, upshifted the current-voltage curve of ICa-L, and shifted the activation and inactivation curves of ICa-L leftward.
Moreover, CBG diminished the amplitude of the cell shortening and Ca2+ transients with a decrease in the time to peak (Tp) and the time to 50% of the baseline (Tr).
CBG inhibited L-type Ca2+ channels, and reduced [Ca2+]i and contractility in adult rat ventricular myocytes.
These findings contribute to the understanding of the cardioprotective efficacy of CBG.
American Psychological Association (APA)
Li, Pinya& Song, Qiongtao& Liu, Tao& Wu, Zhonglin& Chu, Xi& Zhang, Xuan…[et al.]. 2014. Inhibitory Effect of Cinobufagin on L-Type Ca2+ Currents, Contractility, and Ca2+ Homeostasis of Isolated Adult Rat Ventricular Myocytes. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1049845
Modern Language Association (MLA)
Li, Pinya…[et al.]. Inhibitory Effect of Cinobufagin on L-Type Ca2+ Currents, Contractility, and Ca2+ Homeostasis of Isolated Adult Rat Ventricular Myocytes. The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1049845
American Medical Association (AMA)
Li, Pinya& Song, Qiongtao& Liu, Tao& Wu, Zhonglin& Chu, Xi& Zhang, Xuan…[et al.]. Inhibitory Effect of Cinobufagin on L-Type Ca2+ Currents, Contractility, and Ca2+ Homeostasis of Isolated Adult Rat Ventricular Myocytes. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1049845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049845