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HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora
Joint Authors
Wang, M.-C.
Yen, Feng-Lin
Lee, Chiang-Wen
Ko, Horng-Huey
Lin, Fang-Ju
Chen, Pei-Chun
Lin, Chun-Nan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Phyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions.
It has been used as a folk medicine, herbal beverage, or folk cosmetic.
For these usages, the development of a chemical quality control method of this plant is necessary.
In the present study, ten compounds, namely, 3,7,4′,5′-tetrahydroxy-3′-methoxyflavone (1), nodifloretin (2), 4′-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4′-tetrahydroxy-3′-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P.
nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature.
The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems.
Compounds 4, 5, and 7 showed strong antioxidant activity.
To control the quality of P.
nodiflora, a simple and reliable method of high-performance liquid chromatography combined with ultraviolet detector (HPLC-UV) was established for both the fingerprint analysis and the quantitative determination of two selected active compounds, onopordin (4) and eupafolin (7).
Statistical analysis of the obtained data demonstrated that our method achieved the desired linearity, precision, and accuracy.
The results indicated that the developed method can be used as a quality evaluation method for PNM.
American Psychological Association (APA)
Lin, Fang-Ju& Yen, Feng-Lin& Chen, Pei-Chun& Wang, M.-C.& Lin, Chun-Nan& Lee, Chiang-Wen…[et al.]. 2014. HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049988
Modern Language Association (MLA)
Lin, Fang-Ju…[et al.]. HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1049988
American Medical Association (AMA)
Lin, Fang-Ju& Yen, Feng-Lin& Chen, Pei-Chun& Wang, M.-C.& Lin, Chun-Nan& Lee, Chiang-Wen…[et al.]. HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1049988
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1049988