![](/images/graphics-bg.png)
Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions
Joint Authors
Gu, Chun-Sun
Liu, Liang-qin
Xu, Chen
Zhao, Yan-hai
Zhu, Xu-dong
Huang, Su-Zhen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression.
However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples.
In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1α), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), in Iris.
lactea var.
chinensis (I.
lactea var.
chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions.
All seven reference genes showed a relatively wide range of threshold cycles (Ct) values in different samples.
GeNorm and NormFinder algorithms were used to assess the suitable reference genes.
The results from the two software units showed that EIF-5A and UBC were the most stable reference genes across all of the tested samples, while TUBLIN was unsuitable as internal controls.
I.
lactea var.
chinensis is tolerant to Cd, Pb, and salt.
Our results will benefit future research on gene expression in response to the three abiotic stresses.
American Psychological Association (APA)
Gu, Chun-Sun& Liu, Liang-qin& Xu, Chen& Zhao, Yan-hai& Zhu, Xu-dong& Huang, Su-Zhen. 2014. Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1050002
Modern Language Association (MLA)
Gu, Chun-Sun…[et al.]. Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1050002
American Medical Association (AMA)
Gu, Chun-Sun& Liu, Liang-qin& Xu, Chen& Zhao, Yan-hai& Zhu, Xu-dong& Huang, Su-Zhen. Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1050002
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050002