Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
Joint Authors
Jang, Jin Woo
Kim, Ju Yeon
Yoon, Jung
Yoon, Soo Young
Cho, Chi Hyun
Han, Eun Taek
An, Seong Soo A.
Lim, Chae Seung
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P.
falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination.
The cultured P.
falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%.
FACS analysis demonstrated a clear separation between P.
falciparum infected and uninfected RBCs.
The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2–37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2–66.0%).
High correlation of measured parasitaemia ( r = 0.98 , P < 0.05 ) was observed between FACS and Giemsa stained microscopic analyses.
The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis.
The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.
American Psychological Association (APA)
Jang, Jin Woo& Kim, Ju Yeon& Yoon, Jung& Yoon, Soo Young& Cho, Chi Hyun& Han, Eun Taek…[et al.]. 2014. Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1050019
Modern Language Association (MLA)
Jang, Jin Woo…[et al.]. Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A. The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1050019
American Medical Association (AMA)
Jang, Jin Woo& Kim, Ju Yeon& Yoon, Jung& Yoon, Soo Young& Cho, Chi Hyun& Han, Eun Taek…[et al.]. Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1050019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050019