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Progesterone Increases Apoptosis and Inversely Decreases Autophagy in Human Hepatoma HA22TVGH Cells Treated with Epirubicin
Joint Authors
Chiu, Chien-Chih
Cheng, Hsiao-Ling
Hsieh, Bau-Shan
Chang, W. C.
Lee, K. T.
Chang, Kee-Lung
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Hepatocellular carcinoma (HCC) is the leading cause of cancer-related deaths worldwide.
Epirubicin can induce intracellular reactive oxygen species and is widely used to treat unresectable HCC.
Progesterone has been found to inhibit the proliferation of hepatoma cells.
This study was designed to test the combined effects of epirubicin and progesterone on human hepatoma cell line, HA22T/VGH.
These cells were treated with different concentrations of epirubicin with or without the coaddition of 30 μM progesterone and then analyzed for apoptosis, autophagy, and expressions of apoptotic-related proteins and multidrug-resistant gene.
Epirubicin treatment dose-dependently inhibited the growth of HA22T/VGH cells.
Addition of 30 μM progesterone, which was inactive alone, augmented the effect of epirubicin on the inhibition of growth of HA22T/VGH cells.
Cotreatment with progesterone enhanced epirubicin-induced apoptosis, as evidenced by greater increase in caspase-3 activity and in the ratio of the apoptosis-regulating protein, Bax/Bcl-XL.
The combination also caused a decrease in autophagy and in the expression of multidrug resistance-related protein 1 mRNA compared to epirubicin alone.
This study shows the epirubicin/progesterone combination was more effective in increasing apoptosis and inversely decreasing autophagy on HA22T/VGH cells treated with epirubicin alone, suggesting that this combination can potentially be used to treat HCC.
American Psychological Association (APA)
Chang, W. C.& Cheng, Hsiao-Ling& Hsieh, Bau-Shan& Chiu, Chien-Chih& Lee, K. T.& Chang, Kee-Lung. 2014. Progesterone Increases Apoptosis and Inversely Decreases Autophagy in Human Hepatoma HA22TVGH Cells Treated with Epirubicin. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050127
Modern Language Association (MLA)
Chang, W. C.…[et al.]. Progesterone Increases Apoptosis and Inversely Decreases Autophagy in Human Hepatoma HA22TVGH Cells Treated with Epirubicin. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1050127
American Medical Association (AMA)
Chang, W. C.& Cheng, Hsiao-Ling& Hsieh, Bau-Shan& Chiu, Chien-Chih& Lee, K. T.& Chang, Kee-Lung. Progesterone Increases Apoptosis and Inversely Decreases Autophagy in Human Hepatoma HA22TVGH Cells Treated with Epirubicin. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050127
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050127