The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions
Joint Authors
Syed, M. A.
Shukor, Mohd Yunus
Sabullah, Mohd Khalizan
Sulaiman, Mohd Rosni
Khalid, Ariff
Ahmad, Siti Aqlima
Shamaan, N. A.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B.
S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23.
The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C.
The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature.
However, ChE activity showed no significant difference when stored at 4.0, 0, and −25°C for 15 days.
Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P.
javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury.
The results showed that P.
javanicus ChE has the potential to be used as a biosensor for the detection of metal ions.
American Psychological Association (APA)
Sabullah, Mohd Khalizan& Sulaiman, Mohd Rosni& Shukor, Mohd Yunus& Syed, M. A.& Shamaan, N. A.& Khalid, Ariff…[et al.]. 2014. The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050145
Modern Language Association (MLA)
Sabullah, Mohd Khalizan…[et al.]. The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1050145
American Medical Association (AMA)
Sabullah, Mohd Khalizan& Sulaiman, Mohd Rosni& Shukor, Mohd Yunus& Syed, M. A.& Shamaan, N. A.& Khalid, Ariff…[et al.]. The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050145
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050145