Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.)
Joint Authors
Choi, Bosung
Cho, Byoung-Kwan
Lim, Hyoun-Sub
Bae, Hanhong
Ghosh, Ritesh
Bae, Hyeun-Jong
Natarajan, Savithiry
Park, Sang Un
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis.
We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), encoding 339 amino acids of 37.37 kDa, with an isoelectric point (pI) of 6.27 (JX524276, HcCCR2).
BLAST result found that it has high homology with other plant CCR orthologs.
Multiple alignment with other plant CCR sequences showed that it contains two highly conserved motifs: NAD(P) binding domain (VTGAGGFIASWMVKLLLEKGY) at N-terminal and probable catalytic domain (NWYCYGK).
According to phylogenetic analysis, it was closely related to CCR sequences of Gossypium hirsutum (ACQ59094) and Populus trichocarpa (CAC07424).
HcCCR2 showed ubiquitous expression in various kenaf tissues and the highest expression was detected in mature flower.
HcCCR2 was expressed differentially in response to various stresses, and the highest expression was observed by drought and NaCl treatments.
American Psychological Association (APA)
Ghosh, Ritesh& Choi, Bosung& Cho, Byoung-Kwan& Lim, Hyoun-Sub& Park, Sang Un& Bae, Hanhong…[et al.]. 2014. Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.). The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1050279
Modern Language Association (MLA)
Bae, Hanhong…[et al.]. Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.). The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1050279
American Medical Association (AMA)
Ghosh, Ritesh& Choi, Bosung& Cho, Byoung-Kwan& Lim, Hyoun-Sub& Park, Sang Un& Bae, Hanhong…[et al.]. Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.). The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1050279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050279