Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential
Joint Authors
Sasikumar, Ponnusamy
Gomathi, Sivasamy
Anbazhagan, Kolandaswamy
Selvam, Govindan Sadasivam
Sasikumar, Sundaresan
Kavitha, Murugan
Selvi, M. S.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Lactic acid bacteria (LAB) have the potential to degrade intestinal oxalate and this is increasingly being studied as a promising probiotic solution to manage kidney stone disease.
In this study, oxalate degrading LAB were isolated from human faeces and south Indian fermented foods, subsequently assessed for potential probiotic property in vitro and in vivo.
Based on preliminary characteristics, 251 out of 673 bacterial isolates were identified as LAB.
A total of 17 strains were found to degrade oxalate significantly between 40.38% and 62.90% and were subjected to acid and bile tolerance test.
Among them, nine strains exhibited considerable tolerance up to pH 3.0 and at 0.3% bile.
These were identified as Lactobacillus fermentum and Lactobacillus salivarius using 16S rDNA sequencing.
Three strains, Lactobacillus fermentum TY5, Lactobacillus fermentum AB1, and Lactobacillus salivarius AB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity.
They also conferred resistance to kanamycin, rifampicin, and ampicillin, but were sensitive to chloramphenicol and erythromycin.
The faecal recovery rate of these strains was observed as 15.16% (TY5), 6.71% (AB1), and 9.3% (AB11) which indicates the colonization ability.
In conclusion, three efficient oxalate degrading LAB were identified and their safety assessments suggest that they may serve as good probiotic candidates for preventing hyperoxaluria.
American Psychological Association (APA)
Gomathi, Sivasamy& Sasikumar, Ponnusamy& Anbazhagan, Kolandaswamy& Sasikumar, Sundaresan& Kavitha, Murugan& Selvi, M. S.…[et al.]. 2014. Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050492
Modern Language Association (MLA)
Gomathi, Sivasamy…[et al.]. Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1050492
American Medical Association (AMA)
Gomathi, Sivasamy& Sasikumar, Ponnusamy& Anbazhagan, Kolandaswamy& Sasikumar, Sundaresan& Kavitha, Murugan& Selvi, M. S.…[et al.]. Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050492