Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro
Joint Authors
Taha, Rosna Mat
Khorasani Esmaeili, Arash
Mohajer, Minoo
Mohajer, Sadegh
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
To explore the potential of in vitro rapid regeneration, three varieties (Golpaygan-181, Orumieh-1763, and Gorgan-1601) of sainfoin (Onobrychis viciifolia Scop.
syn.
Onobrychis sativa L.) were evaluated.
For the first time, an encapsulation protocol was established from somatic embryogenic callus in torpedo and cotyledonary stages to create artificial seeds.
Callus derived from different concentrations of Kinetin (0–2.0 mg L−1) and Indole-3-acetic acid (0–2.0 mg L−1) was coated with sodium alginate and subsequently cultured either in Murashige and Skoog (MS) medium or in soil substrate.
Adventitious shoots from synthetic beads developed into rooting in full and half strength MS medium supplemented with various concentrations of auxin and cytokinin.
Prolonged water conservation of black and red soils (1 : 1) had the highest rate of survival plantlets in the acclimatization process.
Diverse resistance techniques in Onobrychis viciifolia were evaluated when the plants were subjected to water deficiency.
Higher frequency of epicuticular waxes was observed in in vivo leaves compared to in vitro leaves.
Jagged trichomes nonsecreting glands covered by spines were only observed in the lower leaf side.
Ultimately, stomata indices were 0.127 (abaxial), 0.188 (adaxial) in in vivo and 0.121 (abaxial), 0.201 (adaxial) in in vitro leaves.
American Psychological Association (APA)
Mohajer, Sadegh& Taha, Rosna Mat& Mohajer, Minoo& Khorasani Esmaeili, Arash. 2014. Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1050584
Modern Language Association (MLA)
Mohajer, Sadegh…[et al.]. Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro. The Scientific World Journal No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1050584
American Medical Association (AMA)
Mohajer, Sadegh& Taha, Rosna Mat& Mohajer, Minoo& Khorasani Esmaeili, Arash. Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1050584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050584