In Vitro Acaricidal Activity of Atropa belladonna and Its Components, Scopolamine and Atropine, against Rhipicephalus (Boophilus)‎ microplus

Abstract EN

In vitro efficacy of methanolic extract of Atropa belladonna and its components scopolamine and atropine was assessed against Rhipicephalus (Boophilus) microplus.

Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) were used whereas scopolamine and atropine were each tested at 0.1%.

In adult immersion test, the extract was lethal to ticks at 20% concentration.

The LC50 and LC95 values were determined as 6.875% and 17.306%, respectively.

The extract caused a significant reduction (P<0.05) in egg mass production at 10% concentration.

In larval packet test, the extract was lethal to larvae in the concentrations of 10% and 20% after 24 h, with LC50 and LC95 values of 1.321% and 4.935%, respectively.

Scopolamine and atropine showed 93.3% and 60.0% mortality of adult ticks, respectively, but they caused complete (100%) blocking of hatching as well as 100% larval mortality.

Scopolamine and atropine were observed to be more potent than the crude extract at an equivalent concentration in both the bioassays.