Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
Joint Authors
Zhu, Xing-Quan
Zheng, Xu
Chang, Qiao-Cheng
Zhang, Yan
Tian, Si-Qin
Lou, Yan
Duan, Hong
Guo, Dong-Hui
Wang, Chun-Ren
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time.
The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2), the 28S rDNA, and the intergenic spacer (IGS) regions, had a length range of 8,493–10,221 bp.
The lengths of the investigated 18S, ITS1, 5.8S, ITS2, and 28S rDNA sequences, which were 1,994 bp, 1,293 bp, 157 bp, 286 bp, and 4,186 bp, respectively, did not vary.
However, the IGS rDNA sequences had a length range of 577–2,305 bp.
The 5.8S and ITS-2 rDNA sequences had 100% identity among the five investigated samples, while the identities among the IGS had a range of 53.7–99.8%.
A comparative analysis revealed that different types and numbers of repeats were found within each ITS1 and IGS region, which may be related to the length polymorphism of IGS.
The phylogenetic position of P.
cervi in Paramphistomatidae was analyzed based on the 18S rDNA sequences.
These results will aid in studying the intra- and interspecific variation of the Paramphistomatidae and the systematics and phylogenetics of Digenea.
American Psychological Association (APA)
Zheng, Xu& Chang, Qiao-Cheng& Zhang, Yan& Tian, Si-Qin& Lou, Yan& Duan, Hong…[et al.]. 2014. Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050907
Modern Language Association (MLA)
Zheng, Xu…[et al.]. Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1050907
American Medical Association (AMA)
Zheng, Xu& Chang, Qiao-Cheng& Zhang, Yan& Tian, Si-Qin& Lou, Yan& Duan, Hong…[et al.]. Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050907