A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4
Joint Authors
Liang, Shih-Shin
Kuo, Chao-Jen
Lin, Yi-Reng
Lin, Hugo You-Hsien
Wu, Chin-Jen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
We synthesized unmodified Fe3O4 nanoparticles (NPs) with particles size from 10 nm to 100 nm.
We cultured NRK-52E cell lines (rat, kidney) and treated with Fe3O4 NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells.
Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins.
From the statistical data of identified proteins, we believed that NPs treatment causes cell death and promotes expression of ras-related proteins.
In order to avoid apoptosis, NRK-52E cell lines induce a series of protective effects such as glutathione related proteins to reduce reactive oxygen species (ROS), and chaperone proteins to recycle damaged proteins.
We suggested that, in the indigenous cellular environment, Fe3O4 NPs treatment induced an antagonistic effect for cell lines to go to which avoids apoptosis.
American Psychological Association (APA)
Lin, Yi-Reng& Kuo, Chao-Jen& Lin, Hugo You-Hsien& Wu, Chin-Jen& Liang, Shih-Shin. 2014. A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050923
Modern Language Association (MLA)
Lin, Yi-Reng…[et al.]. A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1050923
American Medical Association (AMA)
Lin, Yi-Reng& Kuo, Chao-Jen& Lin, Hugo You-Hsien& Wu, Chin-Jen& Liang, Shih-Shin. A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050923
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050923