Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity

Joint Authors

Fen, Li
Xuwei, Zhu
Nanyi, Li
Puyu, Zhang
Shuang, Zhang
Xue, Zhao
Pengju, Li
Qichao, Zhu
Haiping, Lin

Source

The Scientific World Journal

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-02-12

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Medicine
Information Technology and Computer Science

Abstract EN

In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase).

The results showed that the ratio of transparent circle to mycelium circle of Hericium erinaceus was 8.16 (P<0.01) higher than other strains.

The filter paper culture screening test showed that Hericium erinaceus and Macrolepiota procera grew well and showed extreme decomposition of the filter paper.

When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm.

CMCase activity determination indicated that Coprinus comatus and Hericium erinaceus had the ability to produce CMCase with 33.92 U/L on the 9th day and 22.58 U/L on the 10th day, respectively, while Coprinus comatus and Pleurotus nebrodensis had the ability to produce laccase with 496.67 U/L and 489.17 U/L on the 16th day and 18th day.

Based on the results, Coprinus comatus might be the most promising lignocellulose-degrading strain to produce both CMCase and laccase at high levels.

American Psychological Association (APA)

Fen, Li& Xuwei, Zhu& Nanyi, Li& Puyu, Zhang& Shuang, Zhang& Xue, Zhao…[et al.]. 2014. Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1050958

Modern Language Association (MLA)

Fen, Li…[et al.]. Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity. The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1050958

American Medical Association (AMA)

Fen, Li& Xuwei, Zhu& Nanyi, Li& Puyu, Zhang& Shuang, Zhang& Xue, Zhao…[et al.]. Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1050958

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1050958