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Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal
Joint Authors
Amador, Paula
Oliveira, Carla
Prudêncio, Cristina
Fernandes, R.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Extended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains.
In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%).
β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches.
TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%).
Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime.
Of the 193 isolates 81.3% presented transferable plasmids harboring blaESBL genes.
Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences.
This study reports a high diversity of genetic patterns.
Ten clusters were found for E.
coli isolates and five clusters for K.
pneumoniae strains by means of ERIC analysis.
In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.
American Psychological Association (APA)
Fernandes, R.& Amador, Paula& Oliveira, Carla& Prudêncio, Cristina. 2014. Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051009
Modern Language Association (MLA)
Fernandes, R.…[et al.]. Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal. The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1051009
American Medical Association (AMA)
Fernandes, R.& Amador, Paula& Oliveira, Carla& Prudêncio, Cristina. Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051009