Molecular Divergence and Species Delimitation of the Cultivated Oyster Mushrooms: Integration of IGS1 and ITS
Joint Authors
Tan, Yee-Shin
Bhassu, Subha
Avin, Farhat Ahmadi
Shahbazi, Pedram
Sabaratnam, Vikineswary
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Identification of edible mushrooms particularly Pleurotus genus has been restricted due to various obstacles.
The present study attempted to use the combination of two variable regions of IGS1 and ITS for classifying the economically cultivated Pleurotus species.
Integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation.
Phylogenetic tree (IGS1 + ITS) showed seven distinct clades, each clade belonging to a separate species group.
Moreover, the species differentiation was tested by AMOVA and the results were reconfirmed by presenting appropriate amounts of divergence (91.82% among and 8.18% within the species).
In spite of achieving a proper classification of species by combination of IGS1 and ITS sequences, the phylogenetic tree showed the misclassification of the species of P.
nebrodensis and P.
eryngii var.
ferulae with other strains of P.
eryngii.
However, the constructed median joining (MJ) network could not only differentiate between these species but also offer a profound perception of the species’ evolutionary process.
Eventually, due to the sufficient variation among and within species, distinct sequences, simple amplification, and location between ideal conserved ribosomal genes, the integration of IGS1 and ITS sequences is recommended as a desirable DNA barcode.
American Psychological Association (APA)
Avin, Farhat Ahmadi& Bhassu, Subha& Tan, Yee-Shin& Shahbazi, Pedram& Sabaratnam, Vikineswary. 2014. Molecular Divergence and Species Delimitation of the Cultivated Oyster Mushrooms: Integration of IGS1 and ITS. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051049
Modern Language Association (MLA)
Avin, Farhat Ahmadi…[et al.]. Molecular Divergence and Species Delimitation of the Cultivated Oyster Mushrooms: Integration of IGS1 and ITS. The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1051049
American Medical Association (AMA)
Avin, Farhat Ahmadi& Bhassu, Subha& Tan, Yee-Shin& Shahbazi, Pedram& Sabaratnam, Vikineswary. Molecular Divergence and Species Delimitation of the Cultivated Oyster Mushrooms: Integration of IGS1 and ITS. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051049