Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro
Joint Authors
Chang, Zhiqiang
Huo, Lifeng
Li, Kun
Wu, Yiming
Hu, Zhenming
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The adjuvant chemotherapy, such as cisplatin, doxorubicin, and methotrexate has significantly improved survival of osteosarcoma patients.
However, the chemoresistance which arose with the chemotherapy blocks achieving favorable outcomes for some patients and finally led to relapse or metastatic disease.
Studies have shown paradoxical functions of autophagy in tumor development, which has been demonstrated by microRNAs.
In the present study, we determined the involvement of autophagy during the chemotherapy of osteosarcoma cell line, U-2 OS, and further determined the regulation of miR-101 on the autophagy in the U-2 OS cells.
Results demonstrated that doxorubicin treatment of U-2 OS cells induced significantly high level of autophagy-characteristic acidic vesicular organelles (AVOs), and induced significant autophagy related protein expression in U-2 OS cells.
While the miR-101 could significantly reduce the doxorubicin-induced AVOs and block the autophagy related protein expression in U-2 OS cells.
Moreover, the autophagy blockage by miR-101 sensitized the U-2 OS cells to doxorubicin treatment.
In summary, miR-101 blocks autophagy during the chemotherapy in osteosarcoma cells and enhances chemosensitivity in vitro.
American Psychological Association (APA)
Chang, Zhiqiang& Huo, Lifeng& Li, Kun& Wu, Yiming& Hu, Zhenming. 2014. Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051059
Modern Language Association (MLA)
Chang, Zhiqiang…[et al.]. Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1051059
American Medical Association (AMA)
Chang, Zhiqiang& Huo, Lifeng& Li, Kun& Wu, Yiming& Hu, Zhenming. Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051059