Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination
Joint Authors
Wu, Yu-Ching
Chen, Ruei-Feng
Liu, Chia-Yang
Hu, Fung Rong
Huang, Chang-Jen
Wang, I. J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Cell polarity during eye development determines the normal retinal lamination and differentiation of photoreceptor cells in the retina.
In vertebrates, blood vessel epicardial substance (Bves) is known to play an important role in the formation and maintenance of the tight junctions essential for epithelial cell polarity.
In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination.
Immunostaining with different specific antibodies from retinal cells and transmission electron microscopy were used to identify the morphological defects in normal and Bves knockdown zebrafish.
In normal zebrafish, Bves is located at the apical junctions of embryonic retinal neuroepithelia during retinogenesis; later, it is strongly expressed around inner plexiform layer (IPL) and retinal pigment epithelium (RPE).
In contrast, a loss of normal retinal lamination and cellular polarity was found with undifferentiated photoreceptor cells in Bves knockdown zebrafish.
Herein, our results indicated that disruption of Bves will result in a loss of normal retinal lamination.
American Psychological Association (APA)
Wu, Yu-Ching& Chen, Ruei-Feng& Liu, Chia-Yang& Hu, Fung Rong& Huang, Chang-Jen& Wang, I. J.. 2014. Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051097
Modern Language Association (MLA)
Wu, Yu-Ching…[et al.]. Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1051097
American Medical Association (AMA)
Wu, Yu-Ching& Chen, Ruei-Feng& Liu, Chia-Yang& Hu, Fung Rong& Huang, Chang-Jen& Wang, I. J.. Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051097
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051097