Fluoroscopy Assisted Minimally Invasive Transplantation of Allogenic Mesenchymal Stromal Cells Embedded in HyStem Reduces the Progression of Nucleus Pulposus Degeneration in the Damaged Interverbal Disc: A Preliminary Study in Rabbits
Joint Authors
Raghavendran, H. Balaji
Kamarul, T.
Subhan, Rifa Aquidah
Karunanithi, Puvanan
Malliga Raman, Murali
Samuel, Shani
Abdullah, Basri Johan Jeet
Abbas, Azlina Amir
Mohamed, Jamal Azmi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
This study was conducted to develop a technique for minimally invasive and accurate delivery of stem cells to augment nucleus pulposus (NP) in damaged intervertebral discs (IVD).
IVD damage was created in noncontiguous discs at L4-L5 level; rabbits ( N = 12 ) were randomly divided into three groups: group I treated with MSCs in HyStem hydrogel, group II treated with HyStem alone, and group III received no intervention.
MSCs and hydrogel were administered to the damaged disc under guidance of fluoroscopy.
Augmentation of NP was assessed through histological and MRI T2 mapping of the NP after eight weeks of transplantation.
T2 weighted signal intensity was higher in group I than in groups II and III ( P < 0.05 ).
Disc height index showed maximum disc height in group I compared to groups II and III.
Histological score of the degenerative index was significantly ( P < 0.05 ) lower in group I (8.6 ± 1.8) than that in groups II (11.6 ± 2.3) and III (18.0 ± 5.7).
Immunohistochemistry staining for collagen type II and aggrecan staining were higher in group I as compared to other groups.
Our results demonstrate that the minimally invasive administration of MSCs in hyaluronan hydrogel (HyStem) augments the repair of NP in damaged IVD.
American Psychological Association (APA)
Subhan, Rifa Aquidah& Karunanithi, Puvanan& Malliga Raman, Murali& Raghavendran, H. Balaji& Samuel, Shani& Abdullah, Basri Johan Jeet…[et al.]. 2014. Fluoroscopy Assisted Minimally Invasive Transplantation of Allogenic Mesenchymal Stromal Cells Embedded in HyStem Reduces the Progression of Nucleus Pulposus Degeneration in the Damaged Interverbal Disc: A Preliminary Study in Rabbits. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1051187
Modern Language Association (MLA)
Subhan, Rifa Aquidah…[et al.]. Fluoroscopy Assisted Minimally Invasive Transplantation of Allogenic Mesenchymal Stromal Cells Embedded in HyStem Reduces the Progression of Nucleus Pulposus Degeneration in the Damaged Interverbal Disc: A Preliminary Study in Rabbits. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1051187
American Medical Association (AMA)
Subhan, Rifa Aquidah& Karunanithi, Puvanan& Malliga Raman, Murali& Raghavendran, H. Balaji& Samuel, Shani& Abdullah, Basri Johan Jeet…[et al.]. Fluoroscopy Assisted Minimally Invasive Transplantation of Allogenic Mesenchymal Stromal Cells Embedded in HyStem Reduces the Progression of Nucleus Pulposus Degeneration in the Damaged Interverbal Disc: A Preliminary Study in Rabbits. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1051187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051187