The Connection between the Toxicity of Anthracyclines and Their Ability to Modulate the P-Glycoprotein-Mediated Transport in A549, HepG2, and MCF-7 Cells
Joint Authors
Rogalska, Aneta
Szwed, Marzena
Rychlik, Błażej
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Multidrug resistance (MDR) is a major obstacle to the successful chemotherapy of solid tumors.
We compared the resistance of the most popular solid tumors, breast adenocarcinoma (MCF-7 cell line) and nonsmall cell lung (A549 cell line) hepatocellular liver carcinoma (HepG2 cells), to aclarubicin (ACL) and doxorubicin (DOX).
This research aimed at determining the relation between the toxicity of ACL and DOX, their cell accumulation, and then effect on P-glycoprotein functionality.
ACL is more cytotoxic for tumor cells compared to DOX.
The intracellular concentration of drugs in cancer cells was dependent on the dose of the drugs and the time of incubation.
The P-gp inhibitor Verapamil (V) increased DOX accumulation in all tested cell lines.
By contrast, the intracellular level of ACL was not affected by this modifying agent.
The assessment of the uptake of 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) or Rhodamine 123 (R123) allows the evaluation of the different influence of drugs on P-gp activity which is in agreement with the estimation of expression measured by MDR-1 shift assay.
These data suggest that ACL is less P-gp dependent than DOX and consequently may be used in a clinical setting to increase treatment efficacy in resistant human tumors.
American Psychological Association (APA)
Rogalska, Aneta& Szwed, Marzena& Rychlik, Błażej. 2014. The Connection between the Toxicity of Anthracyclines and Their Ability to Modulate the P-Glycoprotein-Mediated Transport in A549, HepG2, and MCF-7 Cells. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051193
Modern Language Association (MLA)
Rogalska, Aneta…[et al.]. The Connection between the Toxicity of Anthracyclines and Their Ability to Modulate the P-Glycoprotein-Mediated Transport in A549, HepG2, and MCF-7 Cells. The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1051193
American Medical Association (AMA)
Rogalska, Aneta& Szwed, Marzena& Rychlik, Błażej. The Connection between the Toxicity of Anthracyclines and Their Ability to Modulate the P-Glycoprotein-Mediated Transport in A549, HepG2, and MCF-7 Cells. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051193
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051193