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A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli
Joint Authors
Elloumi-Mseddi, Jihene
Jellali, Karim
Aifa, Sami
Villalobo, Antonio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-15
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Our success in producing an active epidermal growth factor receptor (EGFR) tyrosine kinase in Escherichia coli encouraged us to express the full-length receptor in the same host.
Despite its large size, we were successful at producing the full-length EGFR protein fused to glutathione S-transferase (GST) that was detected by Western blot analysis.
Moreover, we obtained a majoritarian truncated GST-EGFR form detectable by gel electrophoresis and Western blot.
This truncated protein was purified and confirmed by MALDI-TOF/TOF analysis to belong to the N-terminal extracellular region of the EGFR fused to GST.
Northern blot analysis showed two transcripts suggesting the occurrence of a transcriptional arrest.
American Psychological Association (APA)
Elloumi-Mseddi, Jihene& Jellali, Karim& Villalobo, Antonio& Aifa, Sami. 2014. A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051244
Modern Language Association (MLA)
Elloumi-Mseddi, Jihene…[et al.]. A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli. The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1051244
American Medical Association (AMA)
Elloumi-Mseddi, Jihene& Jellali, Karim& Villalobo, Antonio& Aifa, Sami. A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051244
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051244