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Comparison of Microtox and Xenoassay Light as a Near Real Time River Monitoring Assay for Heavy Metals
Joint Authors
Halmi, M. I. E.
Shukor, Mohd Yunus
Johari, W. L. W.
Jirangon, H.
Syed, M. A.
Abdul Rachman, A. R.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Luminescence-based assays for toxicants such as Microtox, ToxAlert, and Biotox have been used extensively worldwide.
However, the use of these assays in near real time conditions is limited due to nonoptimal assay temperature for the tropical climate.
An isolate that exhibits a high luminescence activity in a broad range of temperatures was successfully isolated from the mackerel, Rastrelliger kanagurta.
This isolate was tentatively identified as Photobacterium sp.
strain MIE, based on partial 16S rDNA molecular phylogeny.
Optimum conditions that support high bioluminescence activity occurred between 24 and 30°C, with pH 5.5 to 7.5, 10 to 20 g/L of sodium chloride, 30 to 50 g/L of tryptone, and 4 g/L of glycerol as the carbon source.
Assessment of near real time capability of this bacterial system, Xenoassay light to monitor heavy metals from a contaminated river running through the Juru River Basin shows near real time capability with assaying time of less than 30 minutes per samples.
Samples returned to the lab were tested with a standard Microtox assay using Vibrio fishceri.
Similar results were obtained to Xenoassay light that show temporal variation of copper concentration.
Thus, this strain is suitable for near real time river monitoring of toxicants especially in the tropics.
American Psychological Association (APA)
Halmi, M. I. E.& Jirangon, H.& Johari, W. L. W.& Abdul Rachman, A. R.& Shukor, Mohd Yunus& Syed, M. A.. 2014. Comparison of Microtox and Xenoassay Light as a Near Real Time River Monitoring Assay for Heavy Metals. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051256
Modern Language Association (MLA)
Halmi, M. I. E.…[et al.]. Comparison of Microtox and Xenoassay Light as a Near Real Time River Monitoring Assay for Heavy Metals. The Scientific World Journal No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1051256
American Medical Association (AMA)
Halmi, M. I. E.& Jirangon, H.& Johari, W. L. W.& Abdul Rachman, A. R.& Shukor, Mohd Yunus& Syed, M. A.. Comparison of Microtox and Xenoassay Light as a Near Real Time River Monitoring Assay for Heavy Metals. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051256