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Functional Characterization of Sesquiterpene Synthase from Polygonum minus
Joint Authors
Zainal, Zamri
Shaharuddin, N. A.
Ee, Su-Fang
Mohamed-Hussein, Zeti-Azura
Othman, Roohaida
Ismail, Ismanizan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Polygonum minus is an aromatic plant, which contains high abundance of terpenoids, especially the sesquiterpenes C15H24.
Sesquiterpenes were believed to contribute to the many useful biological properties in plants.
This study aimed to functionally characterize a full length sesquiterpene synthase gene from P.
minus.
P.
minus sesquiterpene synthase (PmSTS) has a complete open reading frame (ORF) of 1689 base pairs encoding a 562 amino acid protein.
Similar to other sesquiterpene synthases, PmSTS has two large domains: the N-terminal domain and the C-terminal metal-binding domain.
It also consists of three conserved motifs: the DDXXD, NSE/DTE, and RXR.
A three-dimensional protein model for PmSTS built clearly distinguished the two main domains, where conserved motifs were highlighted.
We also constructed a phylogenetic tree, which showed that PmSTS belongs to the angiosperm sesquiterpene synthase subfamily Tps-a.
To examine the function of PmSTS, we expressed this gene in Arabidopsis thaliana.
Two transgenic lines, designated as OE3 and OE7, were further characterized, both molecularly and functionally.
The transgenic plants demonstrated smaller basal rosette leaves, shorter and fewer flowering stems, and fewer seeds compared to wild type plants.
Gas chromatography-mass spectrometry analysis of the transgenic plants showed that PmSTS was responsible for the production of β-sesquiphellandrene.
American Psychological Association (APA)
Ee, Su-Fang& Mohamed-Hussein, Zeti-Azura& Othman, Roohaida& Shaharuddin, N. A.& Ismail, Ismanizan& Zainal, Zamri. 2014. Functional Characterization of Sesquiterpene Synthase from Polygonum minus. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1051294
Modern Language Association (MLA)
Ee, Su-Fang…[et al.]. Functional Characterization of Sesquiterpene Synthase from Polygonum minus. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1051294
American Medical Association (AMA)
Ee, Su-Fang& Mohamed-Hussein, Zeti-Azura& Othman, Roohaida& Shaharuddin, N. A.& Ismail, Ismanizan& Zainal, Zamri. Functional Characterization of Sesquiterpene Synthase from Polygonum minus. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1051294
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051294