Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis
Joint Authors
Liu, Guo-Hua
Zhu, Xing-Quan
Cai, Xian-Quan
Yu, Hai-Qiong
Li, Rong
Yue, Qiao-Yun
Bai, Jian-Shan
Deng, Yan
Qiu, De-Yi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Clonorchis sinensis and Opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in Asia.
The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis for the specific detection and rapid identification of C.
sinensis and O.
viverrini.
Primers targeting COX1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes.
Assays using genomic DNA extracted from the two flukes yielded specific amplification and their identity was confirmed by sequencing, having the accuracy of 100% in reference to conventional methods.
The assay was proved to be highly sensitive with a detection limit below 1 pg of purified genomic DNA, 5 EPG, or 1 metacercaria of C.
sinensis.
Moreover, C.
sinensis and O.
viverrini were able to be differentiated by their HRM profiles.
The method can reduce labor of microscopic examination and the contamination of agarose electrophoresis.
Moreover, it can differentiate these two flukes which are difficult to be distinguished using other methods.
The established method provides an alternative tool for rapid, simple, and duplex detection of C.
sinensis and O.
viverrini.
American Psychological Association (APA)
Cai, Xian-Quan& Yu, Hai-Qiong& Li, Rong& Yue, Qiao-Yun& Liu, Guo-Hua& Bai, Jian-Shan…[et al.]. 2014. Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051486
Modern Language Association (MLA)
Cai, Xian-Quan…[et al.]. Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis. The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1051486
American Medical Association (AMA)
Cai, Xian-Quan& Yu, Hai-Qiong& Li, Rong& Yue, Qiao-Yun& Liu, Guo-Hua& Bai, Jian-Shan…[et al.]. Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051486
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051486