Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli

Abstract EN

A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity.

The actinomycete was subjected to submerged fermentation by “shake flask” method.

The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min.

The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time.

Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8.

The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.