Genome-Wide Characterisation of Gene Expression in Rice Leaf Blades at 25°C and 30°C
Joint Authors
Zhou, Jianhua
Shen, Haihong
E, Zhi-guo
Wang, Lei
Qin, Ryan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Rice growth is greatly affected by temperature.
To examine how temperature influences gene expression in rice on a genome-wide basis, we utilised recently compiled next-generation sequencing datasets and characterised a number of RNA-sequence transcriptome samples in rice seedling leaf blades at 25°C and 30°C.
Our analysis indicated that 50.4% of all genes in the rice genome (28,296/56,143) were expressed in rice samples grown at 25°C, whereas slightly fewer genes (50.2%; 28,189/56,143) were expressed in rice leaf blades grown at 30°C.
Among the genes that were expressed, approximately 3% were highly expressed, whereas approximately 65% had low levels of expression.
Further examination demonstrated that 821 genes had a twofold or higher increase in expression and that 553 genes had a twofold or greater decrease in expression at 25°C.
Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested that the ribosome pathway and multiple metabolic pathways were upregulated at 25°C.
Based on these results, we deduced that gene expression at both transcriptional and translational levels was stimulated at 25°C, perhaps in response to a suboptimal temperature condition.
Finally, we observed that temperature markedly regulates several super-families of transcription factors, including bZIP, MYB, and WRKY.
American Psychological Association (APA)
E, Zhi-guo& Wang, Lei& Qin, Ryan& Shen, Haihong& Zhou, Jianhua. 2014. Genome-Wide Characterisation of Gene Expression in Rice Leaf Blades at 25°C and 30°C. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1051591
Modern Language Association (MLA)
E, Zhi-guo…[et al.]. Genome-Wide Characterisation of Gene Expression in Rice Leaf Blades at 25°C and 30°C. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1051591
American Medical Association (AMA)
E, Zhi-guo& Wang, Lei& Qin, Ryan& Shen, Haihong& Zhou, Jianhua. Genome-Wide Characterisation of Gene Expression in Rice Leaf Blades at 25°C and 30°C. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1051591
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051591