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Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping
Joint Authors
Deng, B.
Li, X. F.
Chen, D. Y.
You, L. D.
Wang, J. B.
Chen, J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis.
However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization.
To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper.
By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices.
Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency.
Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments.
Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency.
By fine tuning parameters, more than 95% of trapping sites were taken by individual cells.
This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.
American Psychological Association (APA)
Deng, B.& Li, X. F.& Chen, D. Y.& You, L. D.& Wang, J. B.& Chen, J.. 2014. Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051641
Modern Language Association (MLA)
Deng, B.…[et al.]. Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1051641
American Medical Association (AMA)
Deng, B.& Li, X. F.& Chen, D. Y.& You, L. D.& Wang, J. B.& Chen, J.. Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051641
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051641