The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Aktβ-Catenin-Foxo3A Axis
Joint Authors
Wang, Hui-Min
Chiu, Chien-Chih
Fong, Yao
Lin, Yin-Chieh
Wu, Chang-Yi
Lin, Li-Li
Chou, Han Lin
Teng, Yen-Ni
Yuan, Shyng Shiou
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Sirtuins, NAD+-dependent deacetylases, could target both histones and nonhistone proteins in mammalian cells.
Sirt1 is the major sirtuin and has been shown to involve various cellular processes, including antiapoptosis, cellular senescence.
Sirt1 was reported to be overexpressed in many cancers, including lung cancer.
Sirtinol, a specific inhibitor of Sirt1, has been shown to induce apoptosis of cancer cells by elevating endogenous level of reactive oxygen species.
In the study, we investigated the effect of sirtinol on the proliferation and apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells.
The results of proliferation assay and colony formation assay showed the antigrowth effect of sirtinol.
The annexin-V staining further confirmed the apoptosis induction by sirtinol treatment.
Interestingly, the levels of phosphorylated Akt and β-catenin were significantly downregulated with treating the apoptotic inducing doses.
On the contrary, sirtinol treatment causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1.
These above results suggested that sirtinol may inhibit cell proliferation of H1299 cells by regulating the axis of Akt-β-catenin-FoxO3a.
Overall, this study demonstrates that sirtinol attenuates the proliferation and induces apoptosis of NSCLC cells, indicating the potential treatment against NSCLC cells by inhibiting Sirt1 in future applications.
American Psychological Association (APA)
Fong, Yao& Lin, Yin-Chieh& Wu, Chang-Yi& Wang, Hui-Min& Lin, Li-Li& Chou, Han Lin…[et al.]. 2014. The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Aktβ-Catenin-Foxo3A Axis. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051665
Modern Language Association (MLA)
Fong, Yao…[et al.]. The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Aktβ-Catenin-Foxo3A Axis. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1051665
American Medical Association (AMA)
Fong, Yao& Lin, Yin-Chieh& Wu, Chang-Yi& Wang, Hui-Min& Lin, Li-Li& Chou, Han Lin…[et al.]. The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Aktβ-Catenin-Foxo3A Axis. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051665
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051665